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Hu W, Ding Y, Li Q, Shi R, He Y (2020) Transient receptor potential vanilloid4 channels as therapeutic targets in diabetes and diabetes-related complications

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Crystallization of Pt GSTF8 in presence of ()-catechin and GSH Purified Pt GSTF8 in 30 mM Tris, pH 8, 200 mM NaCl, 1 mM EDTA was concentrated to 12.4 mg ml 1 using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and supplemented with 1 mM GSH and 25 mM ()-catechin immediately before crystallization

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